Dr. Tobias Brügmann

Institute of Forest Genetics
Sieker Landstraße 222927 Großhansdorf
- Telephone
- +49 4102 696 170
- Fax
- +49 4102 696 200
- tobias.bruegmann@thuenen.de
Head of Junior Research Group "Genetic Technologies"
Scientific projects at the Thünen Institute
Since 2021: Head of the Junior Research Group "Genetic Technologies"
2018-2021: Project Scientist in "aProPop" (Development of a DNA-free genome editing system in poplar by transient Cas9/gRNA plasmid and ribonucleinprotein transfer into protoplasts and germinating pollen)
2012-2017: Project Scientist at the Thünen Institute of Forest Genetics in the project "PopMass" (Development and use of novel gene technologies to increase biomass yield in the woody perennial Populus spec.)
The Junior Research Group is funded by the Federal Ministry of Food and Agriculture (BMEL) via Fachagentur für Nachwachsende Rohstoffe (FNR).
All other projects were funded by the Federal Federal Ministry of Education and Research (BMBF).
CV
Promotion 2017: "Genetische Modifikation von SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1), FRUITFULL (FUL) und weiterer Kandidatengene in Pappelhybriden (Populus spec.)" (final grade "mit Auszeichnung/summa cum laude"; supervisors PD Dr. M. Fladung & Prof. Dr. H.-P. Mühlbach, co-supervisor PD Dr. D. Warnecke)
Since 2011: Master of Science, Biology (Molecular Biology and Biotechnology)
2011: Master thesis "Entwicklung molekularer Marker zur genetischen Charakterisierung unterschiedlicher Genotypen der Gattung Populus" ("Development of molecular markers for genetic characterization of different genotypes from the genus Populus")
2009: Bachelor thesis "Optimierung von RNA-Extraktion und cDNA-Synthese in Quercus robur" ("Optimization of RNA extraction and cDNA synthesis for Quercus robur")
2006-2011: Study of biology, University of Hamburg, Germany
University Teaching
and further responsibilities
Lecturer at the University of Hamburg
- Winter semester 2023/24: Lecture "Plant Biotechnology" in the Master's programme in Biology, module leader Dr Tobias Brügmann
- Summer semester 2023: Seminar "Plant Biotechnology" in the Master's degree programme in Biology, module leader Dr Tobias Brügmann
- Summer semester 2022: "Molecular Biology and Genetics" practical course in the Bachelor's degree programme in Biology, module leader Prof. Dr Julia Kehr
Core Group member of the EU COST-Action "CopyTree", Webinar Coordinator
Certificate of competence for project managers and persons responsible for biological safety according to §15 Gentechnik-Sicherheitsverordnung (GenTSV)
Master Thesis Sarah Wodtke (2024) - ongoing
Course Biology, University of Hamburg
Master Thesis Anke Bollen (2023/2024) - ongoing
Course Applied Environmental Sciences, University of Koblenz
Master Thesis Felix Wiedemann (2023/2024) - ongoing
Course Biotechnology, University of Hannover
Master Thesis Friederike Schwarzer (2023/2024)
Course Bioinformatics, TU Braunschweig
Bachelor Thesis Ina Martini (2023)
Course Biotechnology, Hochschule Emden-Leer
Bachelor Thesis Alina Fomin (2022/2023)
Course Biochemistry, University of Lübeck
Bachelor Thesis Keelin Meyer (2022/2023)
Course Biotechnology, Hochschule Emden-Leer
Bachelor Thesis Thalia von Nethen (2021/2022)
Course Biotechnology, Hochschule Emden-Leer
Bachelor Thesis Stefan Zebbedies (2020/2021)
Course Technology of Renewable Resources, Hochschule Hannover
Bachelor Thesis Oliver Sulkowski (2019/2020)
Course Bioinformatics/Biotechnology, Hochschule Emden-Leer
Bachelor Thesis Jakob Fromme (2017)
Course Biotechnology, Hochschule für angewandte Wissenschaften
Publications
- 0
Zahn V, Fendel A, Sievers A-J, Fladung M, Brügmann T (2025) Benefiting from the past: establishing in vitro culture of European beech (Fagus sylvatica L.) from provenance trial trees and seedlings. Plant Methods 21:31, DOI:10.1186/s13007-025-01350-3
- 1
Wodtke S, Fendel A, Brügmann T (2025) Brush them away : a simple method to remove trichomes and visualize stomata. Mar 24, 2025. Berkeley: protocols io, DOI:10.17504/protocols.io.kxygxwjqwv8j/v1
- 2
Grünhofer P, Herzig L, Zhang Q, Vitt S, Stöcker T, Malkowsky Y, Brügmann T, Fladung M, Schreiber L (2024) Changes in wax composition but not amount enhance cuticular transpiration. Plant Cell Environ 47(1):91-105, DOI:10.1111/pce.14719
- 3
Brügmann T, Fendel A, Zahn V, Fladung M (2024) Genome editing in forest trees. In: Ricroch A, Eriksson D, Miladinovic D, Sweet JB, Laere K van, Wozniak-Gientka E (eds) A roadmap for plant genome editing. Cham: Springer, pp 347-372, DOI:10.1007/978-3-031-46150-7_20
- 4
Brügmann T, Fladung M (2024) Genscheren in der Forstpflanzenzüchtung? AFZ Der Wald 79(5):27-30
- 5
Brügmann T, Orgel F, Jelkmann S, Schröder H (2024) Isolation of high quality RNA from tree leaves using Polyclar in the Spectrum Plant Total RNA Kit. Oct 08, 2024. Berkeley: protocols io, DOI:10.17504/protocols.io.14egn6qkml5d/v1
- 6
Rupps A, Thiesen T, Raschke J, Walther M, Ehmke E, Safranek L, Eckardt J, Schmidt A, Beck W, Bubner B, Eisold A-M, Zahn V, Fendel A, Brügmann T (2024) PInK-NET: Resuming the network idea of in vitro tree labs in Germany. In: Sota V, Werbrouck S (eds) In vitro culture of woody crops: problem solving by new approaches : The 2nd Conference of Cost Action CA21157 CopyTree, 22-24 April, 2024, Bulduri Technical School, Jurmala, Latvia ; Book of proceedings. pp 221-230
- 7
Cardi T, Murovec J, Bakhsh A, Boniecka J, Brügmann T, Bull SE, Eeckhaut T, Fladung M, Galovic V, Linkiewicz A, Lukan T, Mafra I, Michalski K, Kavas M, Nicolia A, Nowakowska J, Sagi L, Sarmiento C, Yildirim K, Zlatkovic M, et al (2023) CRISPR/Cas-mediated plant genome editing: outstanding challenges a decade after implementation. Trends Plant Sci 28(10):1144-1165, DOI:10.1016/j.tplants.2023.05.012
- 8
Shahzaib M, Brügmann T, Shakeel M, Khan SH, Azhar MT, Atif RM, Fladung M, Rana IA (2023) Development of climate smart fruit plants via CRISPR/Cas genome editing systems: A spatiotemporal review [Preprint]. 2023051887. Basel: Preprints, 22 p, DOI:10.20944/preprints202305.1887.v1
- 9
Zahn V, Sievers A-J, Fladung M, Brügmann T (2023) Etablierung einer stabilen in vitro-Kultur für Fagus sylvatica - Ein erster Schritt hin zur Genomeditierung in Buchen. Thünen Rep 105:9-10