Project
Genome analysis in trees
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Structural and functional analysis of genomes of forest trees
Trees are characterized by a number of specific characteristics which are under genetic control, like longevity and wood formation.
Background and Objective
It is expected that several genes are involved in the mentioned processes. The aim of this task is the isolation and characterization of regulatory and other different gene sequences which are specific for trees. Species of the genus Populus (poplar) will be used for the investigations.
Approach
Poplars are an excepted model system for trees because of their relatively small genome and accessibility to the Agrobacterium transformation system. Following Arabidopsis and rice the genome of Populus has been totally sequenced. These information serve as basis for the planned investigations.
Preliminary Results
The strategy is based on results of earlier work. Here it was shown for the first time that the genetic transformation of poplar using the transposon Ac from maize is feasible. The results show that Ac excises from its original position in the construct and re-integrates somewhere in the genome (transposition). Further it was demonstrated, that transposition of Ac still occurs in six-years old transgenic plants.; ; Following transformation of Ac more than 100 different, independent transgenic aspe
Thünen-Contact
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Involved Thünen-Partners
Duration
Permanent task 2.2000
More Information
Project status:
ongoing
Publications
- 0
Wehenkel C, Hernández-Díaz JC, Hernández-Velasco J, Simental-Rodríguez SL, Porth I, Goessen R, González-Elizondo MS, Fladung M, Groppe K, Jaramillo-Correa JP, Olivas-García JM, Gernandt DS, Martínez-Ávalos JG, Carrillo-Parra A, Mendoza-Maya E, Sáenz-Romero C, Blanco-García A (2024) Mexican Populus tremuloides Michx: Adaptation to climate change under extreme heterozygote excess. In: Porth I, Klápšte J, McKown A (eds) The poplar genome. Cham: Springer, pp 183-190, DOI:10.1007/978-3-031-50787-8_9
- 1
Mosca E, Cruz F, Gomez-Garrido J, Bianco L, Rellstab C, Brodbeck S, Csillery K, Fady B, Fladung M, Fussi B, Gömöry D, González-Martínez SC, Grivet D, Gut M, Hansen OK, Heer K, Kaya Z, Krutovsky KV, Kersten B, Liepelt S, et al (2019) A reference genome sequence for the European silver fir (Abies alba Mill.): A community-generated genomic resource. G3 Genes Genomes Genetics 9(7):2039-2049, DOI:10.1534/g3.119.400083
- 2
Hernández-Velasco J, Hernández-Díaz JC, Fladung M, Cañadas-Lopez A, Prieto-Ruiz JA, Wehenkel C (2017) Spatial genetic structure in four Pinus species in the Sierra Madre Occidental, Durango, Mexico. Can J Forest Res 47:73-80, DOI:10.1139/cjfr-2016-0154
- 3
Ortiz-Olivas ME, Hernández-Díaz JC, Fladung M, Cañadas-Lopez A, Prieto-Ruiz JA, Wehenkel C (2017) Spatial genetic structure within and among seed stands of Pinus engelmannii Carr. and Pinus leiophylla Schiede ex Schltdl. & Cham, in Durango, Mexico. Forests 8:22, DOI:10.3390/f8010022
- 4
Kersten B, Faivre Rampant P, Mader M, Le Paslier M-C, Bounon R, Berard A, Vettori C, Schröder H, Leplé J-C, Fladung M (2016) Genome sequences of Populus tremula chloroplast and mitochondrion: Implications for holistic poplar breeding. PLoS One 11(1):e0147209, DOI:10.1371/journal.pone.0147209
- 5
Mader M, Le Paslier M-C, Bounon R, Berard A, Faivre Rampant P, Fladung M, Leplé J-C, Kersten B (2016) Whole-genome draft assembly of Populus tremula x P. alba clone INRA 717-1B4. Silvae Genetica 65(2):74-79, DOI:10.1515/sg-2016-0019
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Pakull B, Kersten B, Lüneburg J, Fladung M (2015) A simple PCR-based marker to determine sex in aspen. Plant Biol 17(1):256-261, DOI:10.1111/plb.12217
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Kersten B, Voß M-M, Fladung M (2015) Development of mitochondrial SNP markers in different Populus species. Trees 29(2):575-582, DOI:10.1007/s00468-014-1136-5
- 8
Fladung M (2014) Prospects of using a modified Ac/Ds transposon system from maize for activation tagging in the tree species Populus. In: Ramawat KG, Mérillon J-M, Ahuja MR (eds) Tree biotechnology. Boca Raton: CRC Press ; Taylor & Francis, pp 469-482
- 9
Kersten B, Pakull B, Groppe K, Lüneburg J, Fladung M (2014) The sex-linked region in Populus tremuloides Turesson 141 corresponds to a pericentromeric region of about two million base pairs on P. trichocarpa chromosome 19. Plant Biol 16(2):411-418, DOI:10.1111/plb.12048